Hotspot

atpF-atpA

LSC · 28025-28100 · 76 bp

Back to Ligularia

Pi 0.0124

MarkerSeek score 54.10

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: LSC
length_bp: 76
variable_sites: 3
pi: 0.0124
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	ATATTGGCATATTGGGGGCG	GTCGGCTTGAATGGTTACCA	50.0	50.0	58.1	58.2	134-140 bp	1.0	81.4
atpF-atpA_p2	ATATTGGCATATTGGGGGCG	ATTTCGTCGGCTTGAATGGT	50.0	45.0	58.1	58.2	139-145 bp	1.0	81.3
atpF-atpA_p3	ATATTGGCATATTGGGGGCG	TCGTCGGCTTGAATGGTTAC	50.0	50.0	58.1	58.3	136-142 bp	1.0	80.6
atpF-atpA_p4	ATATTGGCATATTGGGGGCG	CAATGCCGTCACCTACTTGA	50.0	50.0	58.1	57.9	231-237 bp	1.0	80.5
atpF-atpA_p5	ATATTGGCATATTGGGGGCG	GCTCAATACGTTCGCGGATA	50.0	50.0	58.1	58.2	168-174 bp	1.0	80.2