Hotspot

atpI-atpH

LSC · 25033-26127 · 1095 bp

Back to Ligularia

Pi 0.00112

MarkerSeek score 47.90

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1095
variable_sites: 4
pi: 0.00112
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TTTTTGCAACTTTAGCCGCG	ATAACGGAAGCGGCAGAAAT	45.0	45.0	58.5	58.0	1180-1206 bp	1.0	76.2
atpI-atpH_p2	TTTTTGCAACTTTAGCCGCG	TACCTTGACCAACTCCAGGT	45.0	50.0	58.5	57.9	1236-1262 bp	1.0	75.6
atpI-atpH_p3	TTTTTGCAACTTTAGCCGCG	AGCCAATCCAGCAGCAATAA	45.0	45.0	58.5	57.9	1196-1222 bp	1.0	75.3
atpI-atpH_p4	TCAGTGGTTCCTATCCCTGT	ATAACGGAAGCGGCAGAAAT	50.0	45.0	57.4	58.0	1241-1267 bp	1.0	75.2
atpI-atpH_p5	TTTTTGCAACTTTAGCCGCG	AATAACGGAAGCGGCAGAAA	45.0	45.0	58.5	58.2	1181-1207 bp	1.0	75.1