Hotspot

trnC-GCA-petN

LSC · 9442-10251 · 810 bp

Back to Ligularia

Pi 0.00893

MarkerSeek score 58.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 20
pi: 0.00893
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GTTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.0	58.2	916-928 bp	1.0	82.2
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	859-871 bp	1.0	82.1
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	877-889 bp	1.0	79.8
trnC-GCA-petN_p4	TGTTGTGTATCGTTTTGGCG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.3	58.2	917-929 bp	1.0	77.1
trnC-GCA-petN_p5	GTTGTGTATCGTTTTGGCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.0	57.1	915-927 bp	1.0	76.3