Hotspot

ndhA

SSC · 123307-125453 · 2147 bp

Back to Licuala

Pi 0.00349

MarkerSeek score 60.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2147
variable_sites: 18
pi: 0.00349
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTCACCATAGAGAACA	GTCCACCCGGTTTCATCAAT	47.6	50.0	58.1	58.2	2373-2382 bp	1.0	70.9
ndhA_p2	TGAACCCAGTCACCATAGAGA	GTCCACCCGGTTTCATCAAT	47.6	50.0	57.8	58.2	2376-2385 bp	1.0	70.2
ndhA_p3	ACCCAGTCACCATAGAGAACA	TCCACCCGGTTTCATCAATT	47.6	45.0	58.1	57.4	2372-2381 bp	1.0	67.4
ndhA_p4	TGAACCCAGTCACCATAGAGA	TCCACCCGGTTTCATCAATT	47.6	45.0	57.8	57.4	2375-2384 bp	1.0	66.7
ndhA_p5	ACCCAGTCACCATAGAGAACA	CCCGGTTTCATCAATTTGCA	47.6	45.0	58.1	57.3	2368-2377 bp	1.0	66.3