Hotspot

rpl32-trnL-UAG

SSC · 116471-117252 · 782 bp

Back to Licuala

Pi 0.0132
MarkerSeek score 58.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
782
variable_sites
23
pi
0.0132
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTTTCTACCGGACATTCAA AGCGTGTCTACCGATTTCAC 45.0 50.0 55.4 58.0 872-888 bp 1.0 62.2
rpl32-trnL-UAG_p2 GGTTTCTACCGGACATTCAA CAGCGTGTCTACCGATTTCA 45.0 50.0 55.4 58.0 873-889 bp 1.0 62.2
rpl32-trnL-UAG_p3 GGTTTCTACCGGACATTCAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 55.4 57.9 882-898 bp 1.0 61.3
rpl32-trnL-UAG_p4 GGTTTCTACCGGACATTCAA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 55.4 57.8 881-897 bp 1.0 60.7
rpl32-trnL-UAG_p5 GGTTTCTACCGGACATTCAA CTAAGAGCAGCGTGTCTACC 45.0 55.0 55.4 57.8 880-896 bp 1.0 60.7