Hotspot

ndhF-rpl32

SSC · 115899-116296 · 398 bp

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Pi 0.0279

MarkerSeek score 59.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 398
variable_sites: 19
pi: 0.0279
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AATCGGTCGTGTGAACCAAA	ACATACCACAGCAGCTAGTTC	45.0	47.6	58.0	57.7	1076-1191 bp	1.0	75.2
ndhF-rpl32_p2	ATCGGTCGTGTGAACCAAAA	ACATACCACAGCAGCTAGTTC	45.0	47.6	58.0	57.7	1075-1190 bp	1.0	75.2
ndhF-rpl32_p3	CATACGTGGAGGGGGAATTG	ACATACCACAGCAGCTAGTTC	55.0	47.6	58.0	57.7	1312-1427 bp	1.0	75.1
ndhF-rpl32_p4	CATACGTGGAGGGGGAATTG	ACCACAGCAGCTAGTTCTAAC	55.0	47.6	58.0	57.7	1308-1423 bp	1.0	74.6
ndhF-rpl32_p5	AGCAACTGCACCGACAAATA	ACATACCACAGCAGCTAGTTC	45.0	47.6	58.1	57.7	1283-1398 bp	1.0	74.5