Hotspot

rps8-rpl14

LSC · 83521-83700 · 180 bp

Back to Licuala

Pi 0.0263

MarkerSeek score 76.30

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 10
pi: 0.0263
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GGCAATAGTGTCCCTACCCA	GAACTCGAGTTTTTGGTGCG	55.0	50.0	58.8	58.0	260-293 bp	1.0	80.4
rps8-rpl14_p2	GGCAATAGTGTCCCTACCCA	GCTCGGGAATTGAGACAGTT	55.0	50.0	58.8	57.9	237-270 bp	1.0	80.0
rps8-rpl14_p3	TCGGCAATAGTGTCCCTACC	GAACTCGAGTTTTTGGTGCG	55.0	50.0	58.9	58.0	262-295 bp	1.0	79.6
rps8-rpl14_p4	GGCAATAGTGTCCCTACCCA	ACTCGAGTTTTTGGTGCGAT	55.0	45.0	58.8	58.1	258-291 bp	1.0	79.6
rps8-rpl14_p5	TCGGCAATAGTGTCCCTACC	GCTCGGGAATTGAGACAGTT	55.0	50.0	58.9	57.9	239-272 bp	1.0	79.2