Hotspot

rpl33-rps18

LSC · 70547-70889 · 343 bp

Back to Licuala

Pi 0.0296

MarkerSeek score 65.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 343
variable_sites: 19
pi: 0.0296
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CAAGCATACGATTCATGGGGA	TAATCGATTCGATCCCCCGA	47.6	50.0	58.2	58.1	434-482 bp	1.0	77.4
rpl33-rps18_p2	AAGCATACGATTCATGGGGA	TAATCGATTCGATCCCCCGA	45.0	50.0	56.7	58.1	433-481 bp	1.0	76.1
rpl33-rps18_p3	AGCATACGATTCATGGGGAA	TAATCGATTCGATCCCCCGA	45.0	50.0	56.7	58.1	432-480 bp	1.0	76.1
rpl33-rps18_p4	CAAGCATACGATTCATGGGG	TAATCGATTCGATCCCCCGA	50.0	50.0	56.6	58.1	434-482 bp	1.0	75.8
rpl33-rps18_p5	ACAAGCATACGATTCATGGGG	TAATCGATTCGATCCCCCGA	47.6	50.0	58.4	58.1	435-483 bp	1.0	75.2