Hotspot

matK-trnK-UUU

LSC · 3253-4103 · 851 bp

Back to Licuala

Pi 0.0273

MarkerSeek score 71.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 42
pi: 0.0273
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTCCTTAAAGAGAAGCGGA	AACGGTAGAGTACTCGGCTT	45.0	50.0	55.9	58.2	927-954 bp	1.0	69.3
matK-trnK-UUU_p2	ACTCCTTAAAGAGAAGCGGA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	55.9	58.2	926-953 bp	1.0	69.3
matK-trnK-UUU_p3	ACTCCTTAAAGAGAAGCGGA	GGGTTGCTAACTCAACGGTA	45.0	50.0	55.9	57.5	940-967 bp	1.0	66.9
matK-trnK-UUU_p4	ACTCCTTAAAGAGAAGCGGA	TTCGGGTTGCTAACTCAACG	45.0	50.0	55.9	58.5	943-970 bp	1.0	66.6
matK-trnK-UUU_p5	ACTCCTTAAAGAGAAGCGGAT	ACGGTAGAGTACTCGGCTTT	42.9	50.0	56.4	58.2	926-953 bp	1.0	65.8