Hotspot

psbE-petL

LSC · 68092-69523 · 1432 bp

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Pi 0.00526

MarkerSeek score 55.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1432
variable_sites: 17
pi: 0.00526
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	ACCAATAAATAGGGCTGACGT	50.0	42.9	57.8	57.1	1531-1555 bp	1.0	64.3
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	ACCAATAAATAGGGCTGACGT	55.0	42.9	58.2	57.1	1526-1550 bp	1.0	63.9
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGACCAATAAATAGGGCTGACG	50.0	45.5	57.8	57.9	1533-1557 bp	1.0	63.1
psbE-petL_p4	ACCTGCAATGAATAGGGACG	ACCAATAAATAGGGCTGACGT	50.0	42.9	57.7	57.1	1606-1630 bp	1.0	62.8
psbE-petL_p5	CTGCAATGAATAGGGACGGT	ACCAATAAATAGGGCTGACGT	50.0	42.9	57.7	57.1	1604-1628 bp	1.0	62.8