Hotspot

accD-psaI

LSC · 61767-62589 · 823 bp

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Pi 0.00397

MarkerSeek score 55.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 8
pi: 0.00397
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAGGGCGTTCTGAATGAGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.4	58.0	986-999 bp	1.0	76.0
accD-psaI_p2	AAGGGCGTTCTGAATGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.4	58.0	985-998 bp	1.0	76.0
accD-psaI_p3	GCTCCACGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.8	58.0	959-972 bp	1.0	74.4
accD-psaI_p4	AAAGGGCGTTCTGAATGAGT	GCCGGAAATACTAACCCCAC	45.0	55.0	57.4	58.3	975-988 bp	1.0	73.6
accD-psaI_p5	AAGGGCGTTCTGAATGAGTT	GCCGGAAATACTAACCCCAC	45.0	55.0	57.4	58.3	974-987 bp	1.0	73.6