Hotspot

ndhC-trnV-UAC

LSC · 53000-54142 · 1143 bp

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Pi 0.00568

MarkerSeek score 44.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1143
variable_sites: 14
pi: 0.00568
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAGAACCA	GCATGTTGGGTCTTTGAAGC	36.4	50.0	55.6	57.9	1256-1265 bp	1.0	46.2
ndhC-trnV-UAC_p2	TCATATTCGTAAAGCAGAACCA	AGAAGGTCTACGGTTCGAGT	36.4	50.0	55.6	57.8	1192-1201 bp	1.0	45.3
ndhC-trnV-UAC_p3	TCATATTCGTAAAGCAGAACCA	TGTTGGGTCTTTGAAGCAGT	36.4	45.0	55.6	57.6	1253-1262 bp	1.0	43.3
ndhC-trnV-UAC_p4	TCATATTCGTAAAGCAGAACCA	GAAGGTCTACGGTTCGAGTC	36.4	55.0	55.6	57.5	1191-1200 bp	1.0	42.5
ndhC-trnV-UAC_p5	TCATATTCGTAAAGCAGAACCAT	GCATGTTGGGTCTTTGAAGC	34.8	50.0	56.1	57.9	1256-1265 bp	1.0	42.4