Hotspot

atpF-atpH

LSC · 13046-13536 · 491 bp

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Pi 0.00672

MarkerSeek score 56.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 491
variable_sites: 8
pi: 0.00672
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GATGGCCAGTGACCAAAAGA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.1	57.3	637-651 bp	1.0	76.5
atpF-atpH_p2	GATGGCCAGTGACCAAAAGA	CGAGGCGGAGGGAAAAATAC	50.0	55.0	58.1	58.7	642-656 bp	1.0	76.4
atpF-atpH_p3	GATGGCCAGTGACCAAAAGA	GAGGCGGAGGGAAAAATACG	50.0	55.0	58.1	58.7	641-655 bp	1.0	76.4
atpF-atpH_p4	GATGGCCAGTGACCAAAAGA	GCGGAGGGAAAAATACGAGG	50.0	55.0	58.1	58.7	638-652 bp	1.0	76.4
atpF-atpH_p5	GATGGCCAGTGACCAAAAGA	GGCGGAGGGAAAAATACGAG	50.0	55.0	58.1	58.7	639-653 bp	1.0	76.4