Hotspot

psbI-trnS-GCU

LSC · 7115-7230 · 116 bp

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Pi 0.0149

MarkerSeek score 57.30

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 116
variable_sites: 4
pi: 0.0149
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TTTATCTAATGACCCGGGGC	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.1	58.0	232-265 bp	1.0	73.8
psbI-trnS-GCU_p2	TTTATCTAATGACCCGGGGC	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.1	58.0	233-266 bp	1.0	73.7
psbI-trnS-GCU_p3	TTTATCTAATGACCCGGGGC	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	251-284 bp	1.0	72.8
psbI-trnS-GCU_p4	TTTATCTAATGACCCGGGGC	CATTGGGAGAGATGGCTGAG	50.0	55.0	57.1	57.7	252-285 bp	1.0	71.6
psbI-trnS-GCU_p5	TTTTATCTAATGACCCGGGGC	TGGACTAAAGCGTCGGATTG	47.6	50.0	57.8	58.0	233-266 bp	1.0	71.6