Hotspot

trnH-GUG-psbA

LSC · 122-533 · 412 bp

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Pi 0.0169

MarkerSeek score 55.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 412
variable_sites: 20
pi: 0.0169
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.2	58.3	474-546 bp	1.0	83.0
trnH-GUG-psbA_p2	CCTTAATCCACTTGGCCACA	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.8	58.3	470-542 bp	1.0	82.6
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	ACCGTGCTAACCTTGGTATG	50.0	50.0	58.2	57.6	475-547 bp	1.0	82.6
trnH-GUG-psbA_p4	CCTTAATCCACTTGGCCACA	ACCGTGCTAACCTTGGTATG	50.0	50.0	57.8	57.6	471-543 bp	1.0	82.1
trnH-GUG-psbA_p5	TAATCCACTTGGCCACATCC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.6	58.3	467-539 bp	1.0	80.9