Hotspot

rps8

LSC · 83168-83572 · 405 bp

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Pi 0

MarkerSeek score 20.90

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 20.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	ATAATTCGTCCGCGTCTACG	GTTTCATTAGCACCCGAGGT	50.0	50.0	58.0	57.9	1228-1273 bp	1.0	83.5
rps8_p2	GTCGAATCCTTCCGGAAACA	TTTACTTCGCCCTTTGCACT	50.0	45.0	57.9	58.0	1327-1372 bp	1.0	83.4
rps8_p3	TGTCGAATCCTTCCGGAAAC	TTTACTTCGCCCTTTGCACT	50.0	45.0	57.9	58.0	1328-1373 bp	1.0	83.4
rps8_p4	ATAATTCGTCCGCGTCTACG	TGATAATGCTGCGGTTGTCA	50.0	45.0	58.0	57.9	1331-1376 bp	1.0	83.2
rps8_p5	ATAATTCGTCCGCGTCTACG	TTTACTTCGCCCTTTGCACT	50.0	45.0	58.0	58.0	1617-1662 bp	1.0	83.1