Hotspot

ccsA-ndhD

SSC · 118630-118915 · 286 bp

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Pi 0.0524

MarkerSeek score 76.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 286
variable_sites: 15
pi: 0.0524
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TCAATAGTGGCCTCTATGGG	CAGTTGACAAGGTCGAAGCT	50.0	50.0	56.1	58.1	432-436 bp	1.0	66.3
ccsA-ndhD_p2	TCAATAGTGGCCTCTATGGG	TCAGTTGACAAGGTCGAAGC	50.0	50.0	56.1	58.1	433-437 bp	1.0	66.3
ccsA-ndhD_p3	CTTCAATAGTGGCCTCTATGGG	CAGTTGACAAGGTCGAAGCT	50.0	50.0	58.0	58.1	434-438 bp	1.0	65.6
ccsA-ndhD_p4	CTTCAATAGTGGCCTCTATGGG	TCAGTTGACAAGGTCGAAGC	50.0	50.0	58.0	58.1	435-439 bp	1.0	65.6
ccsA-ndhD_p5	TTCAATAGTGGCCTCTATGGG	CAGTTGACAAGGTCGAAGCT	47.6	50.0	56.9	58.1	433-437 bp	1.0	64.6