Hotspot

rps11-rpl36

LSC · 82352-82585 · 234 bp

Back to Libidibia

Pi 0.0645

MarkerSeek score 86.70

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 14
pi: 0.0645
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TACGCGCATTCTTACGTGAA	CGTAGACGCGGACGAATTAT	45.0	50.0	58.0	58.0	367-399 bp	1.0	86.1
rps11-rpl36_p2	TTACGCGCATTCTTACGTGA	CGTAGACGCGGACGAATTAT	45.0	50.0	58.0	58.0	368-400 bp	1.0	86.1
rps11-rpl36_p3	CCCTTTGGTATTTTACGCGC	CGTAGACGCGGACGAATTAT	50.0	50.0	57.8	58.0	380-412 bp	1.0	84.6
rps11-rpl36_p4	TACGCGCATTCTTACGTGAA	AAAATAAGGGCCTCCGTTCG	45.0	50.0	58.0	58.3	415-447 bp	1.0	83.6
rps11-rpl36_p5	TTACGCGCATTCTTACGTGA	AAAATAAGGGCCTCCGTTCG	45.0	50.0	58.0	58.3	416-448 bp	1.0	83.6