Hotspot

psbM-trnD-GUC

LSC · 31240-32174 · 935 bp

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Pi 0.0183
MarkerSeek score 69.70
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
935
variable_sites
17
pi
0.0183
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGCAAGAAGGCAGTAGGAAC TTCAATTGGTCAGAGCACCG 50.0 50.0 57.5 58.5 1067-1082 bp 1.0 73.9
psbM-trnD-GUC_p2 GCAAGAAGGCAGTAGGAACT TTCAATTGGTCAGAGCACCG 50.0 50.0 57.5 58.5 1066-1081 bp 1.0 73.9
psbM-trnD-GUC_p3 AGCAAGAAGGCAGTAGGAAC GTTCAATTGGTCAGAGCACC 50.0 50.0 57.5 57.0 1068-1083 bp 1.0 69.7
psbM-trnD-GUC_p4 GCAAGAAGGCAGTAGGAACT GTTCAATTGGTCAGAGCACC 50.0 50.0 57.5 57.0 1067-1082 bp 1.0 69.7
psbM-trnD-GUC_p5 AAGCAAGAAGGCAGTAGGAAC TTCAATTGGTCAGAGCACCG 47.6 50.0 58.2 58.5 1068-1083 bp 1.0 68.0