Hotspot

petN-psbM

LSC · 30248-31134 · 887 bp

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Pi 0.0136

MarkerSeek score 67.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 887
variable_sites: 12
pi: 0.0136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCCTTCTTGC	50.0	50.0	58.2	57.5	1025-1028 bp	1.0	76.3
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	GTTCCTACTGCCTTCTTGCT	50.0	50.0	58.2	57.5	1024-1027 bp	1.0	76.3
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCCTTCTTGC	50.0	50.0	57.6	57.5	964-967 bp	1.0	74.8
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	GTTCCTACTGCCTTCTTGCT	50.0	50.0	57.6	57.5	963-966 bp	1.0	74.8
petN-psbM_p5	CCCTCGTAGTATGGGGAAGA	GTTCCTACTGCCTTCTTGCT	55.0	50.0	57.6	57.5	973-976 bp	1.0	74.5