Hotspot

ycf1

SSC · 108329-113716 · 5388 bp

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Pi 0.00564

MarkerSeek score 46.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5388
variable_sites: 140
pi: 0.00564
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGGATATCGCATTGGTACGC	TCACAGGCTCAGTCCATCTT	50.0	50.0	57.9	58.4	725 bp	1.0	79.8
ycf1_p2	ATGGCGAAAGAGAGTCAACC	TCACAGGCTCAGTCCATCTT	50.0	50.0	57.9	58.4	1055 bp	1.0	79.6
ycf1_p3	GGATATCGCATTGGTACGCA	TCACAGGCTCAGTCCATCTT	50.0	50.0	58.2	58.4	724 bp	1.0	78.7
ycf1_p4	CCGGAAAAATGGCGAAAGAG	TCACAGGCTCAGTCCATCTT	50.0	50.0	57.7	58.4	1063 bp	1.0	78.3
ycf1_p5	GTCAACCGACATCGTACGAT	TCACAGGCTCAGTCCATCTT	50.0	50.0	57.8	58.4	1042 bp	0.923077	74.7