Hotspot

ndhA

SSC · 115717-117892 · 2176 bp

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Pi 0.00465

MarkerSeek score 59.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2176
variable_sites: 48
pi: 0.00465
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCCGCCGGGTTTCATAAATT	AAACCGGTCACCATAGGGAA	45.0	50.0	57.9	58.6	2398-2422 bp	1.0	76.6
ndhA_p2	TCCGCCGGGTTTCATAAATT	ATGAAACCGGTCACCATAGG	45.0	50.0	57.9	57.3	2401-2425 bp	1.0	76.0
ndhA_p3	TCCGCCGGGTTTCATAAATT	AAACCGGTCACCATAGGGA	45.0	52.6	57.9	57.9	2398-2422 bp	1.0	72.9
ndhA_p4	TCCGCCGGGTTTCATAAATT	AACCGGTCACCATAGGGAA	45.0	52.6	57.9	57.9	2397-2421 bp	1.0	72.9
ndhA_p5	TCCGCCGGGTTTCATAAATT	GAAACCGGTCACCATAGGGA	45.0	55.0	57.9	59.1	2399-2423 bp	1.0	72.9