Hotspot

clpP

LSC · 69257-71305 · 2049 bp

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Pi 0.00307

MarkerSeek score 50.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2049
variable_sites: 31
pi: 0.00307
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCGATCTTAACCTGATGATTGA	AAACCATTCTTACGTTTCCA	36.4	35.0	54.7	53.0	2198-2213 bp	1.0	43.0
clpP_p2	AGATCGATCTTAACCTGATGATTGA	AAACCATTCTTACGTTTCCA	36.0	35.0	57.7	53.0	2201-2216 bp	1.0	43.0
clpP_p3	TCGATCTTAACCTGATGATTGATGA	AAACCATTCTTACGTTTCCA	36.0	35.0	57.9	53.0	2200-2213 bp	0.846154	35.1
clpP_p4	TCGATCTTAACCTGATGATTGATGA	AAAACCATTCTTACGTTTCCA	36.0	33.3	57.9	53.9	2201-2214 bp	0.846154	35.1
clpP_p5	TCGATCTTAACCTGATGATTGATGA	AACCATTCTTACGTTTCCAT	36.0	35.0	57.9	52.8	2199-2212 bp	0.846154	35.1