Hotspot

tRNA-Leu-rpl32

SSC · 123378-123993 · 616 bp

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Pi 0.0115

MarkerSeek score 61.10

Primer available yes

Label tRNA-Leu-rpl32

Feature Metadata

label_name: tRNA-Leu-rpl32
feature_type: igs
region: SSC
length_bp: 616
variable_sites: 29
pi: 0.0115
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Leu-rpl32_p1	AGCGTGTCTACCGATTTCAC	AGCTAAATCGGTTTCCACGG	50.0	50.0	58.0	58.3	724-1063 bp	1.0	82.7
tRNA-Leu-rpl32_p2	CAGCGTGTCTACCGATTTCA	AGCTAAATCGGTTTCCACGG	50.0	50.0	58.0	58.3	725-1064 bp	1.0	82.7
tRNA-Leu-rpl32_p3	TCCTAAGAGCAGCGTGTCTA	AGCTAAATCGGTTTCCACGG	50.0	50.0	57.9	58.3	734-1073 bp	1.0	81.7
tRNA-Leu-rpl32_p4	AGCGTGTCTACCGATTTCAC	CTAAATCGGTTTCCACGGGA	50.0	50.0	58.0	57.6	722-1061 bp	1.0	81.7
tRNA-Leu-rpl32_p5	CAGCGTGTCTACCGATTTCA	CTAAATCGGTTTCCACGGGA	50.0	50.0	58.0	57.6	723-1062 bp	1.0	81.7