Hotspot

clpP-psbB

LSC · 71306-71742 · 437 bp

Back to Libertia

Pi 0.032

MarkerSeek score 76.80

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 437
variable_sites: 32
pi: 0.032
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	532-541 bp	1.0	85.9
clpP-psbB_p2	CGCACTATACGTCAACCCAA	TGGACAGAAAGCAATCGACC	50.0	50.0	58.0	58.2	580-589 bp	1.0	85.7
clpP-psbB_p3	CGCACTATACGTCAACCCAA	CGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	58.3	614-623 bp	1.0	84.7
clpP-psbB_p4	CGCACTATACGTCAACCCAA	GTATAGAGCCATCGAACCCG	50.0	55.0	58.0	57.7	632-641 bp	1.0	83.9
clpP-psbB_p5	CGCACTATACGTCAACCCAA	CAATCGACCGGGATCATTCA	50.0	50.0	58.0	57.8	569-578 bp	0.923077	82.0