Hotspot

psbE-petL

LSC · 64408-65360 · 953 bp

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Pi 0.00561

MarkerSeek score 57.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 953
variable_sites: 30
pi: 0.00561
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	GAACGTTCTCCCGTACTTCC	AACCAATCAACAGAGCTGGG	55.0	50.0	58.0	58.1	929-1053 bp	1.0	85.5
psbE-petL_p2	GAACGTTCTCCCGTACTTCC	CAATCAACAGAGCTGGGGTT	55.0	50.0	58.0	58.1	926-1050 bp	1.0	85.5
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AACCAATCAACAGAGCTGGG	50.0	50.0	57.9	58.1	1007-1131 bp	1.0	85.1
psbE-petL_p4	CAACCCGCAATGAATAGGGA	CAATCAACAGAGCTGGGGTT	50.0	50.0	57.9	58.1	1004-1128 bp	1.0	85.1
psbE-petL_p5	ACTGACGAATAACCAACCCG	CAATCAACAGAGCTGGGGTT	50.0	50.0	57.9	58.1	1017-1141 bp	1.0	84.7