Hotspot

tRNA-Phe-ndhJ

LSC · 48586-49289 · 704 bp

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Pi 0.00537

MarkerSeek score 60.50

Primer available yes

Label tRNA-Phe-ndhJ

Feature Metadata

label_name: tRNA-Phe-ndhJ
feature_type: igs
region: LSC
length_bp: 704
variable_sites: 21
pi: 0.00537
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Phe-ndhJ_p1	TGTCACCAGTTCAAACCTGG	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.9	57.9	695-809 bp	1.0	84.7
tRNA-Phe-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	700-814 bp	1.0	84.6
tRNA-Phe-ndhJ_p3	TGTCACCAGTTCAAACCTGG	ATAGGCTGGCCCTTACGTAA	50.0	50.0	57.9	58.2	685-799 bp	1.0	84.1
tRNA-Phe-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	690-804 bp	1.0	84.1
tRNA-Phe-ndhJ_p5	AAGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	742-856 bp	1.0	83.9