Pi
0.00634
MarkerSeek score
58.80
Primer available
yes
Label
tRNA-Thr-tRNA-Leu
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| tRNA-Thr-tRNA-Leu_p1 | CGAACCGATGACCATCACAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 742-948 bp | 1.0 | 85.6 |
| tRNA-Thr-tRNA-Leu_p2 | CGAACCGATGACCATCACAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 750-956 bp | 1.0 | 85.1 |
| tRNA-Thr-tRNA-Leu_p3 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 715-921 bp | 1.0 | 85.1 |
| tRNA-Thr-tRNA-Leu_p4 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 723-929 bp | 1.0 | 84.6 |
| tRNA-Thr-tRNA-Leu_p5 | ATCGAACCGATGACCATCAC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 744-950 bp | 1.0 | 84.3 |