Hotspot

tRNA-Glu-tRNA-Thr

LSC · 31676-32163 · 488 bp

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Pi 0.0108

MarkerSeek score 68.00

Primer available yes

Label tRNA-Glu-tRNA-Thr

Feature Metadata

label_name: tRNA-Glu-tRNA-Thr
feature_type: igs
region: LSC
length_bp: 488
variable_sites: 23
pi: 0.0108
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Glu-tRNA-Thr_p1	CACGATCCACTTCCTTTCGT	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	631-652 bp	1.0	84.8
tRNA-Glu-tRNA-Thr_p2	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	617-638 bp	1.0	84.3
tRNA-Glu-tRNA-Thr_p3	CACGATCCACTTCCTTTCGT	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.9	57.9	596-617 bp	1.0	84.3
tRNA-Glu-tRNA-Thr_p4	TTTCGTAGTACCCTACCCCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.8	57.9	582-603 bp	1.0	83.8
tRNA-Glu-tRNA-Thr_p5	CACGATCCACTTCCTTTCGT	CCGATGACTTACGCCTTACC	50.0	55.0	57.9	58.2	628-649 bp	1.0	83.6