Hotspot

tRNA-Asp-tRNA-Tyr

LSC · 31084-31459 · 376 bp

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Pi 0.00944

MarkerSeek score 43.80

Primer available yes

Label tRNA-Asp-tRNA-Tyr

Feature Metadata

label_name: tRNA-Asp-tRNA-Tyr
feature_type: igs
region: LSC
length_bp: 376
variable_sites: 17
pi: 0.00944
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Asp-tRNA-Tyr_p1	GGATCCTAGTTCGGGACTGA	AATTCTTCCTGGGTCGATGC	55.0	50.0	57.9	58.0	555-570 bp	1.0	81.5
tRNA-Asp-tRNA-Tyr_p2	GGATCCTAGTTCGGGACTGA	GGGGACGGACTGTAAATTCG	55.0	55.0	57.9	58.4	522-537 bp	1.0	79.2
tRNA-Asp-tRNA-Tyr_p3	GGATCCTAGTTCGGGACTGA	GGGACGGACTGTAAATTCGT	55.0	50.0	57.9	57.6	521-536 bp	1.0	79.0
tRNA-Asp-tRNA-Tyr_p4	CGGTGCTCTGACCAATTGAA	AATTCTTCCTGGGTCGATGC	50.0	50.0	58.5	58.0	500-515 bp	1.0	78.5
tRNA-Asp-tRNA-Tyr_p5	GGATCCTAGTTCGGGACTGA	GGACTGTAAATTCGTTGGCG	55.0	50.0	57.9	57.5	516-531 bp	1.0	77.9