Hotspot

petN-psbM

LSC · 28948-29833 · 886 bp

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Pi 0.0054

MarkerSeek score 65.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 886
variable_sites: 25
pi: 0.0054
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	ACGTAAAAACAGTCAGCCAA	55.0	40.0	58.1	55.8	925-960 bp	1.0	65.9
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	ACGTAAAAACAGTCAGCCAA	55.0	40.0	58.1	55.8	924-959 bp	1.0	65.9
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	ACGTAAAAACAGTCAGCCAA	50.0	40.0	58.2	55.8	983-1018 bp	1.0	65.5
petN-psbM_p4	TATGGGGAAGGAGTGGACTC	ACGTAAAAACAGTCAGCCAA	55.0	40.0	57.8	55.8	923-958 bp	1.0	65.5
petN-psbM_p5	GGGGAAGGAGTGGACTCTAG	ACGTAAAAACAGTCAGCCAA	60.0	40.0	58.2	55.8	920-955 bp	1.0	65.2