Hotspot

rpoB-tRNA-Cys

LSC · 26500-27724 · 1225 bp

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Pi 0.0166

MarkerSeek score 76.90

Primer available yes

Label rpoB-tRNA-Cys

Feature Metadata

label_name: rpoB-tRNA-Cys
feature_type: igs
region: LSC
length_bp: 1225
variable_sites: 79
pi: 0.0166
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-tRNA-Cys_p1	CGCCAAGCCCCTATTAATGA	CCGGGGATAAAGGATTTGCA	50.0	50.0	57.7	57.9	1249-1386 bp	1.0	86.0
rpoB-tRNA-Cys_p2	CGCCAAGCCCCTATTAATGA	ACCGGGGATAAAGGATTTGC	50.0	50.0	57.7	57.9	1250-1387 bp	1.0	86.0
rpoB-tRNA-Cys_p3	CGCCAAGCCCCTATTAATGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	57.5	1242-1379 bp	1.0	82.4
rpoB-tRNA-Cys_p4	CGCCAAGCCCCTATTAATGA	CCTTACCACTTGGCCATGTC	50.0	55.0	57.7	58.5	1221-1358 bp	1.0	82.3
rpoB-tRNA-Cys_p5	CCGCCAAGCCCCTATTAATG	CCGGGGATAAAGGATTTGCA	55.0	50.0	58.8	57.9	1250-1387 bp	1.0	82.1