Hotspot

matK-tRNA-Lys

LSC · 3240-4036 · 797 bp

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Pi 0.0064

MarkerSeek score 61.30

Primer available yes

Label matK-tRNA-Lys

Feature Metadata

label_name: matK-tRNA-Lys
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 26
pi: 0.0064
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-tRNA-Lys_p1	ACGATCATGAGCAAGTGCAT	AACGGTAGAGTACTCGGCTT	45.0	50.0	58.0	58.2	921-947 bp	1.0	81.6
matK-tRNA-Lys_p2	ACGATCATGAGCAAGTGCAT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	58.0	58.2	920-946 bp	1.0	81.6
matK-tRNA-Lys_p3	ACGATCATGAGCAAGTGCAT	TTGGGGTTGCTAACTCAACG	45.0	50.0	58.0	58.4	937-963 bp	1.0	79.7
matK-tRNA-Lys_p4	ACCACGATCATGAGCAAGTG	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.3	58.2	924-950 bp	0.923077	76.4
matK-tRNA-Lys_p5	ACCACGATCATGAGCAAGTG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.3	58.2	923-949 bp	0.923077	76.4