Hotspot

ccsA-ndhD

SSC · 117516-117739 · 224 bp

Back to Libanotis

Pi 0.0249

MarkerSeek score 68.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 224
variable_sites: 26
pi: 0.0249
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGATATGCTATTTTGGGGTCA	CAGTTGACAAGGTCGAGGTT	40.9	50.0	57.2	57.8	347-368 bp	1.0	65.1
ccsA-ndhD_p2	TGGATATGCTATTTTGGGGTCA	GTTGACAAGGTCGAGGTTCT	40.9	50.0	57.2	57.5	345-366 bp	1.0	62.9
ccsA-ndhD_p3	TGGATATGCTATTTTGGGGTCA	AGTTGACAAGGTCGAGGTTC	40.9	50.0	57.2	57.5	346-367 bp	1.0	62.8
ccsA-ndhD_p4	TGGATATGCTATTTTGGGGTCAA	CAGTTGACAAGGTCGAGGTT	39.1	50.0	57.9	57.8	347-368 bp	1.0	62.5
ccsA-ndhD_p5	TTGGATATGCTATTTTGGGGTCA	CAGTTGACAAGGTCGAGGTT	39.1	50.0	57.9	57.8	348-369 bp	1.0	62.4