Hotspot

petA-psbJ

LSC · 63628-64586 · 959 bp

Back to Libanotis

Pi 0.0165

MarkerSeek score 78.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 959
variable_sites: 62
pi: 0.0165
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.1	58.1	839-1188 bp	1.0	78.9
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.8	58.1	826-1175 bp	1.0	78.6
petA-psbJ_p3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.1	57.8	840-1189 bp	1.0	78.2
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	57.8	57.8	827-1176 bp	1.0	77.9
petA-psbJ_p5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	55.0	47.6	57.7	58.1	844-1193 bp	1.0	77.3