Hotspot

ycf35

Genome · 9951-10337 · 387 bp

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Pi 0.0672

MarkerSeek score 70.30

Primer available yes

Label ycf35

Feature Metadata

label_name: ycf35
feature_type: gene
region: Genome
length_bp: 387
variable_sites: 26
pi: 0.0672
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf35_p1	GTGATAAAGCTGAAGGCCGA	ATACGATCGTAAGGGCCAGA	50.0	50.0	58.0	58.0	872 bp	0.5	59.7
ycf35_p2	GTGATAAAGCTGAAGGCCGA	CGATCGTAAGGGCCAGAAAA	50.0	50.0	58.0	58.0	869 bp	0.5	59.7
ycf35_p3	GTGATAAAGCTGAAGGCCGA	CATACGATCGTAAGGGCCAG	50.0	55.0	58.0	57.9	873 bp	0.5	59.3
ycf35_p4	GTGGAATTGGTAGACACGCT	ATACGATCGTAAGGGCCAGA	50.0	50.0	57.9	58.0	543 bp	0.5	59.2
ycf35_p5	GTGGAATTGGTAGACACGCT	CGATCGTAAGGGCCAGAAAA	50.0	50.0	57.9	58.0	540 bp	0.5	59.1