Hotspot

chlB

Genome · 116531-119922 · 3392 bp

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Pi 0.0419

MarkerSeek score 64.60

Primer available yes

Label chlB

Feature Metadata

label_name: chlB
feature_type: gene
region: Genome
length_bp: 3392
variable_sites: 142
pi: 0.0419
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB_p1	TTTTCCTGAGAGCCTCTTGC	GCTGCGGAGATCTTATGCTT	50.0	50.0	57.8	58.1	1296 bp	0.5	57.5
chlB_p2	CGGCTTCAAAACCGTACATG	GCTGCGGAGATCTTATGCTT	50.0	50.0	57.8	58.1	1167 bp	0.5	57.1
chlB_p3	CACATCGGACTCTGTTTCCA	GCTGCGGAGATCTTATGCTT	50.0	50.0	57.5	58.1	1438 bp	0.5	55.4
chlB_p4	TCACATCGGACTCTGTTTCC	GCTGCGGAGATCTTATGCTT	50.0	50.0	57.3	58.1	1439 bp	0.5	53.1
chlB_p5	TTTTCCTGAGAGCCTCTTGC	TAGCTGCGGAGATCTTATGC	50.0	50.0	57.8	57.3	1298 bp	0.5	52.6