Hotspot

psbZ-trnG

Genome · 129492-129918 · 427 bp

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Pi 0.101

MarkerSeek score 83.50

Primer available yes

Label psbZ-trnG

Feature Metadata

label_name: psbZ-trnG
feature_type: igs
region: Genome
length_bp: 427
variable_sites: 43
pi: 0.101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG_p1	ATACTGGTTCAGGCCTTTGG	TACCACTGAGCTATACCCGC	50.0	55.0	57.5	58.7	515 bp	1.0	77.2
psbZ-trnG_p2	ATACTGGTTCAGGCCTTTGG	TCTACCACTGAGCTATACCCG	50.0	52.4	57.5	58.1	517 bp	1.0	73.4
psbZ-trnG_p3	ATACTGGTTCAGGCCTTTGG	GTTGCGCTCTACCACTGAG	50.0	57.9	57.5	58.3	524 bp	1.0	72.4
psbZ-trnG_p4	GCATCTCCTGGACAATGGG	TACCACTGAGCTATACCCGC	57.9	55.0	57.9	58.7	555 bp	1.0	71.9
psbZ-trnG_p5	CATCTCCTGGACAATGGGAA	TACCACTGAGCTATACCCGC	50.0	55.0	56.9	58.7	554 bp	1.0	71.9