Hotspot

petN-acsF

Genome · 115064-115408 · 345 bp

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Pi 0.0986

MarkerSeek score 74.80

Primer available yes

Label petN-acsF

Feature Metadata

label_name: petN-acsF
feature_type: igs
region: Genome
length_bp: 345
variable_sites: 34
pi: 0.0986
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-acsF_p1	GGCTGTTGATCAAATGGCAC	GTCTCCAAGCGCCTTGATAA	50.0	50.0	58.0	58.0	1284 bp	0.5	59.8
petN-acsF_p2	GGCTGTTGATCAAATGGCAC	TCCAAGCGCCTTGATAACTC	50.0	50.0	58.0	58.0	1281 bp	0.5	59.8
petN-acsF_p3	GGCTGTTGATCAAATGGCAC	TCTCCAAGCGCCTTGATAAC	50.0	50.0	58.0	58.0	1283 bp	0.5	59.8
petN-acsF_p4	GGCTGTTGATCAAATGGCAC	CCAAGCGCCTTGATAACTCT	50.0	50.0	58.0	58.0	1280 bp	0.5	59.8
petN-acsF_p5	GGCTGTTGATCAAATGGCAC	CTCCAAGCGCCTTGATAACT	50.0	50.0	58.0	58.0	1282 bp	0.5	59.8