Hotspot

rps18-infB

Genome · 97117-97328 · 212 bp

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Pi 0.0955

MarkerSeek score 76.00

Primer available yes

Label rps18-infB

Feature Metadata

label_name: rps18-infB
feature_type: igs
region: Genome
length_bp: 212
variable_sites: 19
pi: 0.0955
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-infB_p1	TTCTGCTGGCCACTGAAATT	GCTGGATGCATGGTTACTGA	45.0	50.0	58.0	58.0	1078-1088 bp	1.0	84.3
rps18-infB_p2	TTTCTGCTGGCCACTGAAAT	GCTGGATGCATGGTTACTGA	45.0	50.0	58.0	58.0	1079-1089 bp	1.0	84.3
rps18-infB_p3	TTCTGCTGGCCACTGAAATT	TGATGATGCTGATGGTTCCG	45.0	50.0	58.0	58.0	1170-1180 bp	1.0	84.2
rps18-infB_p4	ATTTCTGCTGGCCACTGAAA	TGATGATGCTGATGGTTCCG	45.0	50.0	58.0	58.0	1172-1182 bp	1.0	84.1
rps18-infB_p5	TTTCTGCTGGCCACTGAAAT	TGATGATGCTGATGGTTCCG	45.0	50.0	58.0	58.0	1171-1181 bp	1.0	84.1