Hotspot

carA-petJ

Genome · 19778-19889 · 112 bp

Back to Liagora

Pi 0.107

MarkerSeek score 75.90

Primer available yes

Label carA-petJ

Feature Metadata

label_name: carA-petJ
feature_type: igs
region: Genome
length_bp: 112
variable_sites: 12
pi: 0.107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
carA-petJ_p1	TGTTTTATAGGTTTTTGTGTCAAACA	AAGCGGAACAAGGTTGGTAA	26.9	45.0	56.7	57.6	159-165 bp	1.0	45.9
carA-petJ_p2	TGTTTTATAGGTTTTTGTGTCAAACA	CAAGCGGAACAAGGTTGGTA	26.9	50.0	56.7	58.4	160-166 bp	1.0	45.9
carA-petJ_p3	TGTTTTATAGGTTTTTGTGTCAAACA	AAGTCAAGCGGAACAAGGTT	26.9	45.0	56.7	57.9	164-170 bp	1.0	45.7
carA-petJ_p4	TGTTTTATAGGTTTTTGTGTCAAACA	TTGGAGGACGTTTAGAGGCT	26.9	50.0	56.7	58.4	216-222 bp	1.0	44.4
carA-petJ_p5	TGTTTTATAGGTTTTTGTGTCAAACA	TTTGGAGGACGTTTAGAGGC	26.9	50.0	56.7	57.5	217-223 bp	1.0	44.4