Hotspot

infA-rps8

LSC · 76719-76798 · 80 bp

Back to Leymus

Pi 0.0189
MarkerSeek score 53.00
Primer available yes
Label infA-rps8

Feature Metadata

label_name
infA-rps8
feature_type
igs
region
LSC
length_bp
80
variable_sites
5
pi
0.0189
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
infA-rps8_p1 AACATTCCATTGGGTAGGGC GGGTGGAATGGGAATTGCTA 50.0 50.0 57.8 57.5 284-302 bp 1.0 77.1
infA-rps8_p2 GGGTAGGGCTTCCGTAACTA GGGTGGAATGGGAATTGCTA 55.0 50.0 58.2 57.5 273-291 bp 1.0 76.7
infA-rps8_p3 ATTGGGTAGGGCTTCCGTAA GGGTGGAATGGGAATTGCTA 50.0 50.0 58.4 57.5 276-294 bp 1.0 75.1
infA-rps8_p4 AACATTCCATTGGGTAGGGC TTGGGTGGAATGGGAATTGC 50.0 50.0 57.8 58.7 286-304 bp 1.0 74.9
infA-rps8_p5 CATTCCATTGGGTAGGGCTT GGGTGGAATGGGAATTGCTA 50.0 50.0 57.5 57.5 282-300 bp 1.0 74.7