Hotspot

ycf4-cemA

LSC · 58538-59006 · 469 bp

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Pi 0.0248
MarkerSeek score 58.00
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
469
variable_sites
30
pi
0.0248
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.91
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TGCGCGTACCAATTGAAGTA CCCCAAGGCAAAAGTACGAT 45.0 50.0 57.9 58.2 517-560 bp 1.0 86.5
ycf4-cemA_p2 CTTGCGCGTACCAATTGAAG CCCCAAGGCAAAAGTACGAT 50.0 50.0 58.1 58.2 519-562 bp 1.0 86.2
ycf4-cemA_p3 ACAAAAAGCTGCCGAATTGG CCCCAAGGCAAAAGTACGAT 45.0 50.0 57.8 58.2 546-589 bp 1.0 85.7
ycf4-cemA_p4 TTTCTTGCGCGTACCAATTG CCCCAAGGCAAAAGTACGAT 45.0 50.0 57.7 58.2 522-565 bp 1.0 84.5
ycf4-cemA_p5 CCTATTTCTTGCGCGTACCA CCCCAAGGCAAAAGTACGAT 50.0 50.0 58.4 58.2 526-569 bp 1.0 84.3