Hotspot

trnF-GAA-ndhJ

LSC · 47550-48130 · 581 bp

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Pi 0.0191
MarkerSeek score 54.40
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
581
variable_sites
31
pi
0.0191
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.91
markerseek_score
54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA GGATTTGGAGAAGTGCCGAT 50.0 50.0 58.1 58.0 626-805 bp 1.0 86.7
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA CGCGCCTTAAACGTATCCTA 50.0 50.0 58.1 57.9 554-733 bp 1.0 86.3
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA GGCTTTGATCACCGAGGAAT 50.0 50.0 58.1 58.0 873-1052 bp 1.0 85.9
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA TTTACGCTCCCAATGTGCTT 50.0 45.0 58.1 58.1 781-960 bp 1.0 85.8
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA GGGATTGGGATTCCATTGCT 50.0 50.0 58.1 57.9 830-1009 bp 1.0 85.7