Hotspot

ycf3-trnS-GGA

LSC · 43860-44474 · 615 bp

Back to Leymus

Pi 0.00842
MarkerSeek score 54.10
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
615
variable_sites
14
pi
0.00842
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TCTCCTGAGGTTGTCGGAAT ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.1 58.2 798-799 bp 1.0 79.7
ycf3-trnS-GGA_p2 TCTCCTGAGGTTGTCGGAAT TTCCAATGCTACGCCTTGAA 50.0 45.0 58.1 57.8 748-749 bp 1.0 79.5
ycf3-trnS-GGA_p3 TTCTCCTGAGGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.3 58.2 799-800 bp 1.0 77.9
ycf3-trnS-GGA_p4 TTTCTCCTGAGGTTGTCGGA ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.3 58.2 800-801 bp 1.0 77.9
ycf3-trnS-GGA_p5 TTCTCCTGAGGTTGTCGGAA TTCCAATGCTACGCCTTGAA 50.0 45.0 58.3 57.8 749-750 bp 1.0 77.6