Hotspot

trnS-CGA-trnT-GGU

LSC · 13534-14651 · 1118 bp

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Pi 0.0147
MarkerSeek score 59.50
Primer available yes
Label trnS-CGA-trnT-GGU

Feature Metadata

label_name
trnS-CGA-trnT-GGU
feature_type
igs
region
LSC
length_bp
1118
variable_sites
48
pi
0.0147
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-CGA-trnT-GGU_p1 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1129-1320 bp 1.0 47.8
trnS-CGA-trnT-GGU_p2 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1125-1316 bp 1.0 47.0
trnS-CGA-trnT-GGU_p3 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1126-1317 bp 1.0 47.0
trnS-CGA-trnT-GGU_p4 GAACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 1131-1322 bp 1.0 47.0
trnS-CGA-trnT-GGU_p5 ACGAATCACACTTTTACCACT TGACTTACGTCTTAGCACGAG 38.1 47.6 55.5 57.5 1189-1380 bp 1.0 46.9