Hotspot

trnS-GCU-psbD

LSC · 7629-8608 · 980 bp

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Pi 0.0103
MarkerSeek score 53.80
Primer available yes
Label trnS-GCU-psbD

Feature Metadata

label_name
trnS-GCU-psbD
feature_type
igs
region
LSC
length_bp
980
variable_sites
31
pi
0.0103
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.98
markerseek_score
53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-psbD_p1 ATTAGCAATCCGCCGCTTTA TCTACCAAGGGCTATAGTCA 45.0 45.0 58.0 54.3 1036-1090 bp 1.0 54.6
trnS-GCU-psbD_p2 GAGCGGAAAGAGAGGGATTC TCTACCAAGGGCTATAGTCA 55.0 45.0 57.8 54.3 1087-1141 bp 1.0 53.0
trnS-GCU-psbD_p3 ATTAGCAATCCGCCGCTTTA TCTACCAAGGGCTATAGTCAT 45.0 42.9 58.0 55.0 1036-1090 bp 1.0 51.8
trnS-GCU-psbD_p4 GCTTTAGTCCACTCAGCCAT TCTACCAAGGGCTATAGTCA 50.0 45.0 57.6 54.3 1022-1076 bp 1.0 51.7
trnS-GCU-psbD_p5 AGTCCACTCAGCCATCTCTC TCTACCAAGGGCTATAGTCA 55.0 45.0 58.5 54.3 1017-1071 bp 1.0 50.9