Hotspot

ycf1

SSC · 126284-131671 · 5388 bp

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Pi 0.0178

MarkerSeek score 48.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5388
variable_sites: 96
pi: 0.0178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTCGTGCAATGGATCCATCA	ACACCGAAACCCGAATCATT	50.0	45.0	58.3	57.8	1081 bp	1.0	78.4
ycf1_p2	TGTCGTGCAATGGATCCATC	ACACCGAAACCCGAATCATT	50.0	45.0	58.3	57.8	1082 bp	1.0	78.4
ycf1_p3	TCGTGCAATGGATCCATCAA	ACACCGAAACCCGAATCATT	45.0	45.0	57.6	57.8	1080 bp	1.0	77.7
ycf1_p4	GTCGTGCAATGGATCCATCA	CCGTGGATCATCCCAATCAA	50.0	50.0	58.3	57.7	679 bp	1.0	77.5
ycf1_p5	GTCGTGCAATGGATCCATCA	TGCATATCGGCACCAAGATT	50.0	45.0	58.3	57.7	912 bp	1.0	77.1