Hotspot

ndhF

SSC · 112487-114715 · 2229 bp

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Pi 0.0148

MarkerSeek score 51.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 33
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGAATCGCCCGTTGAGATAT	AGCTCATAGTGCCGTGTCTA	50.0	50.0	57.5	58.2	2958-2976 bp	1.0	76.2
ndhF_p2	GGAATCGCCCGTTGAGATAT	TAAGCTCATAGTGCCGTGTC	50.0	50.0	57.5	57.4	2960-2978 bp	1.0	73.4
ndhF_p3	TGGAATCGCCCGTTGAGATA	AGCTCATAGTGCCGTGTCTA	50.0	50.0	58.9	58.2	2959-2977 bp	1.0	72.8
ndhF_p4	GGAATCGCCCGTTGAGATAT	GCTCATAGTGCCGTGTCTAT	50.0	50.0	57.5	57.2	2957-2975 bp	1.0	71.6
ndhF_p5	TGGAATCGCCCGTTGAGATA	TAAGCTCATAGTGCCGTGTC	50.0	50.0	58.9	57.4	2961-2979 bp	1.0	70.0