Hotspot

ndhA

SSC · 122105-124323 · 2219 bp

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Pi 0.0141

MarkerSeek score 66.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2219
variable_sites: 31
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TTTATGAACCCGGTCACCAC	GTCCACCCGGTTTCATCAAT	50.0	50.0	57.8	58.2	2456 bp	1.0	78.8
ndhA_p2	CGGTCACCACAGGAAACATA	GTCCACCCGGTTTCATCAAT	50.0	50.0	57.5	58.2	2446 bp	1.0	76.6
ndhA_p3	TTTATGAACCCGGTCACCAC	TCCACCCGGTTTCATCAATT	50.0	45.0	57.8	57.4	2455 bp	1.0	75.4
ndhA_p4	TTTATGAACCCGGTCACCAC	CCCGGTTTCATCAATTTGCA	50.0	45.0	57.8	57.3	2451 bp	1.0	74.2
ndhA_p5	TTTATGAACCCGGTCACCAC	ACCCGGTTTCATCAATTTGC	50.0	45.0	57.8	57.3	2452 bp	1.0	74.2